Journal: Neural Regeneration Research

Article Title: Srgap2 suppression ameliorates retinal ganglion cell degeneration in mice

doi: 10.4103/1673-5374.369122

Figure Lengend Snippet: Downstream signaling pathways after inhibition of Srgap2 . (A) Detection of SRGAP2 (green, Alexa Fluor 488) expression in PC12 cells by immunofluorescence staining. n = 3 independent cell culture preparations. Scale bars: 50 μm. (B) SRGAP2 protein level was decreased after siRNA transfection and Rac1 and Cdc42 were increased after inhibiting Srgap2. (C) Quantification of B. n = 3 independent cell culture preparations. (D) mTOR signaling pathway was activated after the downregulation of Srgap2. (E) Quantification of D. n = 3 independent cell culture preparations. (F, G) ONC induced activation of the mTOR signaling pathway 7 days after ONC. Srgap2 +/– mice showed increased mTOR activation compared with WT. n = 3 independent animals in each group. Data (normalized by WT-ctrl group) are presented as mean ± SEM. ** P < 0.01, *** P < 0.001, **** P < 0.0001 (two-tailed Student’s t -test). Cdc42: Cell division cycle 42; Ctrl: control; DAPI: 4′,6-diamidino-2-phenylindole; mTOR: mammalian target of rapamycin; ONC: optic nerve crush; Rac1: Ras-related C3 botulinum toxin substrate 1; siRNA: small interfering RNA; Srgap2: Slit-Robo GTPase activating protein 2; WT: wild type.

Article Snippet: PC12, one of the most commonly used cell lines in neuroscience research (You et al., 2022), was purchased from CHI Scientific (Jiangyin, China; RRID: CVCL_0481).

Techniques: Protein-Protein interactions, Inhibition, Expressing, Immunofluorescence, Staining, Cell Culture, Transfection, Activation Assay, Two Tailed Test, Control, Small Interfering RNA

Journal: Cell Death & Disease

Article Title: MiR-127-3p targeting CISD1 regulates autophagy in hypoxic–ischemic cortex

doi: 10.1038/s41419-021-03541-x

Figure Lengend Snippet: A Results of miRNA sequencing analysis showed that the expression of miRNAs in sham and HI have obvious difference ( n = 2/group). B The qRT-PCR validation of differential miRNAs in normal group rats and HI group rats at 12 h after HI injury. Each bar was the mean ± s.d. relative to −log2 (** P < 0.01 with Student’s t -test, n = 6). C Relative expression of miRNA-127-3p in heart, liver, brain, and kidney of rats. MiRNA-127-3p was selectively highly expressed in the brain (** P < 0.01 with one-way ANOVA, n = 6). D qRT-PCR analysis of miR-127-3p expression at 12, 48, and 96 h. MiRNA-127-3p decreased at 12 h after HI, while it increased at 48 and 96 h after HI injury (** P < 0.01 with one-way ANOVA, n = 6). E The relative expression of miR-127-3p in OGD of PC12, SY5Y, and neurons was significantly decreased in vitro (** P < 0.01 with Student’s t -test, n = 6). HI hypoxic ischemic.

Article Snippet: PC12 cell line (GCC-KI0009RT) was obtained from Shanghai Genechem Co., LTD and SY5Y cell line (BFN60700126) was obtained from BLUEFBIO.

Techniques: Sequencing, Expressing, Quantitative RT-PCR, Biomarker Discovery, In Vitro

Journal: Basic and Clinical Neuroscience

Article Title: Effectiveness of Nicotinamide Phosphoribosyltransferase/Pre-B Cell Colony-enhancing Factor/Visfatin in preventing High Glucose-induced Neurotoxicity in an In-vitro Model of Diabetic Neuropathy

doi: 10.32598/bcn.2021.2870.2

Figure Lengend Snippet: A) The effects of different concentrations of glucose on pheochromocytoma PC12 cell viability, B) The effects of visfatin on high-glucose damaged PC12 cells Data are shown as Mean±SEM; * P<0.05, ** P<0.01 vs control, # P<0.001, ## P<0.0001 vs HG treatment.

Article Snippet: PC12 cells were purchased from the Buali (Avicenna) Research Institute.

Techniques: Control

Journal: Basic and Clinical Neuroscience

Article Title: Effectiveness of Nicotinamide Phosphoribosyltransferase/Pre-B Cell Colony-enhancing Factor/Visfatin in preventing High Glucose-induced Neurotoxicity in an In-vitro Model of Diabetic Neuropathy

doi: 10.32598/bcn.2021.2870.2

Figure Lengend Snippet: A) The effect of visfatin on HG-induced intracellular ROS level in PC12 cells, B) The effects of visfatin on HG-induced apoptosis of PC12 cell, C) The apoptosis evaluation of PC12 cells under different treatments Note: Data are shown as Mean±SEM; * P<0.05 vs control, # P<0.01, ## P<0.0001vs HG treatment.

Article Snippet: PC12 cells were purchased from the Buali (Avicenna) Research Institute.

Techniques: Control